Automated nucleic acid extraction – Everything To Know

Don’t just sit there! Let’s learn about the automated nucleic acid extraction system.

Secrets to the automated nucleic acid extraction machine

Researchers from all life science fields use an automated nucleic acid extraction system to start studies in applications ranging from fundamental research to more complicated ones, such as biomarker identification, cell-free diagnostics, epidemiology, and macrobiotics.

Following extraction, nucleic acids are frequently subjected to susceptible quantitative methods such as next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR).

Because of these approaches’ sensitivity, even slight differences or discrepancies in sample preparation, downstream analysis, and automated nucleic acid extraction machines can have a major influence on outcomes, leaving you with potentially unreliable data.

Comparison between Manual &automated nucleic acid extraction system

Manual sample processing is a significant cause of the variance. When working with large sample numbers, this increases the likelihood of pipetting mistakes and protocol deviations.

For example, by the time all samples in a set have finished a particular step, the initial sample has already exceeded the maximum standing time for that step. This circumstance might be crucial when working with delicate nucleic acids like RNA.

Fortunately, you can avoid the dangers of human sample handling by using an automated nucleic acid extraction system. This assures more consistency and dependability, as well as higher-quality data overall.

5 Reasons Why Nucleic Acid Extraction Should Be Automated

The extraction procedure has been streamlined. Pipetting using an automated procedure ensures accuracy and consistency in essential sample processing processes, including lysis, washing, and elution.

Using an automated nucleic acid extraction system will reduce contamination if there is less manipulation. Whether or not the process is carried out in a sterile environment, fewer manual handling and pipetting stages decrease the danger of external contamination and sample carryover.

1. Accurate Research

Using an automated nucleic acid extraction machine, you may process bigger sample numbers at a faster rate, boosting your throughput. This also allows you to extend the scope of your research by experimenting with more test circumstances, medicines, and study participants.

2. Time-Saving

There are two aspects to this. Manual nucleic acid extraction necessitates skilled personnel’s full attention. The researcher may spend more time walking away from the computer while the robot does the monotonous task.

Automated nucleic acid extraction machines are also faster than manual protocols (in terms of time spent on each extraction), allowing you to speed up your process even more.

3. Workflow

In many situations, an automated nucleic acid extraction system can be combined with the desired downstream application(s), for example, automated extraction followed by PCR preparation and purification, resulting in a flawless plate of samples suitable for downstream applications.

Aside from the benefits listed above, an automated nucleic acid extraction machine should make inter-laboratory research easier since the influence of human mistakes and intervention is significantly minimized, resulting in improved data consistency and dependability.

What should you consider when using automated nucleic acid extraction machines?

Firstly, automated DNA extraction systems that use robotic liquid handling technology can reduce the time it takes to extract DNA from a sample by automating activities like serial dilution and cherry-picking. Shaking, temperature control, and PCR procedures are all common features of systems.

Secondly, DNA extraction is utilized in molecular biology, forensics, pathology, environmental studies, and drug development, among other fields of biology. Integration with laboratory information management systems, full start-to-finish automation, error control, and contamination protection are all features to look for when selecting an automated DNA extraction system.

Thirdly, the simplicity of use is the most important factor to consider when purchasing a fully automated nucleic acid extraction machine. While automation saves time and money, it may also add complexity depending on how well it fits into the process.

Humans may adapt procedures and processes to fit their needs when employing automated nucleic acid extraction machines. This facilitates nucleic acid extraction.

According to specialists, many automated DNA extraction systems are worth employing since they make it easier to set up tests in the hopes of getting reliable results.

As a result, any lab apparatus purchase, particularly the usage of a fully automated nucleic acid extraction system, should be chosen with long-term research objectives in mind, such as sample types, potential analytes of interest, and throughput fluctuations.

High-quality nucleic acids must be extracted for sensitive downstream applications such as life sciences, diagnostics, sequencing, forensics, and genetic engineering. As a result, choosing the right automated nucleic acid extraction machine is crucial.

Hence, by opting for an automated DNA extraction machine, you can avoid the problems with manual techniques. An automated nucleic acid extraction machine helps improve consistency, reduces errors, increases speed and throughput, and gives you more time to focus on other exciting lab tasks!

Final Verdict

Congratulations! You have understood everything about automated nucleic acid extraction machines and systems. If you’re thinking about automating your lab, contact us. We’d be happy to guide you through the process.