Rabbits mAb are useful in conducting various research work, making it one of the reasons researchers prefer it as a research tool. They are useful in numerous applications such as ELISA, immunofluorescence, Western Blot, immunohistochemistry, and flow cytometry. They are far better than other animal models such as mice and rats. Researchers prefer a better rabbit since they provide a better system for the production of mAb.

This is due to the rabbit’s immune system that is far better in response to different kinds of antigens. When it comes to other physical advantages, rabbits have a larger spleen, which helps produce more antibodies since they are larger animals themselves. This is why they have been useful in all research work, including Calnexin (C5C9) Rabbit mAb from Cell Signaling Technology. As a result, researchers have produced a host of calnexin antibody products to cover a wide range of research works.

About the product

This product can be applied in different research work. However, here are a few things to know about the product before buying.

Storing this product

The supply of this product comes in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. The product also contains less than 0.02% sodium azide. Storing this product is best at a temperature of –20°C. The following tips should, however, be considered when storing this product

• Do not aliquot the antibody
• Do not expose the product to light
• Avoid freezing this product as this may affect the contents

Specificity / Sensitivity

The Calnexin (C5C9) Rabbit monoclonal antibodies help in detecting endogenous levels of total calnexin protein.

Species Reactivity

This product is reactive to be both human and monkey

Source / Purification

The Calnexin (C5C9) Monoclonal antibody was carried out by immunizing animals with a synthetic peptide corresponding to the sequence of human Calnexin.

Applications

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry E-P-ELISA-Peptide

A Background of Calnexin

The synthesizing of secretory and transmembrane proteins are takes place on polysomes. They are, however, moved into a different position in the endoplasmic reticulum (ER). This is where there is often a modification of the secretory and transmembrane proteins by forming disulphide bonds, amino-linked glycosylation, and folding. For proper folding of proteins, the endoplasmic reticulum has a pool of molecular chaperons and Calnexin. The first identification of Calnexin came as its involvement in the assembly of murine class histocompatibility molecules.

Calnexin is a calcium-binding protein embedded in the endoplasmic reticulum membrane responsible for retaining the newly synthesized glycoproteins inside the endoplasmic reticulum to ensure proper folding and quality control. The specificity of Calnexin for a subset of glycoproteins is defined by a lectin site, which helps in binding an early oligosaccharide intermediate on the folding glycoprotein

Application Key

Due to the wide range of usefulness of rabbit monoclonal antibodies, they remain a better choice for researchers Calnexin (C5C9) Rabbit mAb, making it suitable for different applications. Some of these applications include

1.     WB-Western Blot:

These are important tools used widely for antibody-based techniques for detecting protein expression levels in the extraction of tissue or cell. This technique is accurate for measuring protein levels in a biological sample through antibody binding to certain protein interests.

2.     IP-Immunoprecipitation:

The technique is available for enriching a certain protein from the extraction of heterogeneous cells or tissue using a target-specific antibody. They are employed in the identification of protein-protein interactions, including novel members of protein complexes.

3.     IHC-Immunohistochemistry:

Researchers use this technique to detect and analyze protein expression without losing the composition, cellular characteristics, including the native tissue’s structure. Immunohistochemistry (IHC)is mostly used for the morphological characterization of tumours or other tissue malignancies.

4.     IF-Immunofluorescence:

Researchers use this technique as a method of detection for pairing target-specific antibodies with fluorescent readouts for the production of image data that allows the study of target antigens within the context of intact cells or tissues.

5.     F-Flow Cytometry:

This is a fluorescence-based immunoassay. They help to simultaneously enable the measurement of characteristics of multiple cellular. These include an abundance of protein, population counts from individual cells suspended in a solution. F-Flow cytometry is a powerful tool that accurately, quantitatively, and rapidly measure items during research work. They also help in providing straight insight into heterogeneous cell populations.

• E-P-ELISA-Peptide
• ChIP-Chromatin Immunoprecipitation

Species Cross-Reactivity Key:

H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. Cerevisiae Ce-C. elegans Hr-Horse All-All Species Expected

Product Description

This Cell Signaling Technology antibody, is conjugated to Alexa Fluor® 488 fluorescent dye. This product has been tested in-house for direct immunofluorescent analysis in human cells. However, using this antibody during research work should exhibit the same species cross-reactivity as the unconjugated Calnexin (C5C9) Rabbit mAb #2679.

Conclusion

Rabbits’ monoclonal antibodies have different applications in research work due to its feature. This is why they fit well for Calnexin (C5C9) in carrying out further extensive research work. However, to ensure this product performs at its best, it is important to follow each instruction about storage.

This product is designed for researchers to achieve effective outcomes in research works, making it the reason for validating the antibodies in-house in multiple research applications. Other reasons may be responsible for the products not providing the expected result during research work; however, it is best to follow the storage instructions.